| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067922 |
| Chromosome: | chromosome 17 |
| Location: | 5407464 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738600 | RLS5 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAGACACGCCGGCAAATGTTTTATGCGAAGATGCTGCACGTTGGCCA |
| Internal bar code: | TATTTGTACAAGGCATACGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1301 |
| LEAP-Seq percent confirming: | 21.3333 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCGGGTGTGTAACTTCA |
| Suggested primer 2: | GCTGGAGATCCGCATCATGA |