| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067941 |
| Chromosome: | chromosome 9 |
| Location: | 406162 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749747 | FAP281 | Protein of unknown function; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAAGAGTGCCAACGACTACCGGTATCACAGGTGTGTAGCCAGGTGAA |
| Internal bar code: | TTTAGGTTGCAGTTTAGCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1681 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGGTGTGATGCGTGT |
| Suggested primer 2: | CCGCTGTGGTGCTGTAAAAC |