| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067956 |
| Chromosome: | chromosome 2 |
| Location: | 7227778 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390949 | FAP140 | Flagellar Associated Protein 140 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACAACAGTACAGGGCGAATTGCTTCACTCAGCTCTCCGCTCAGTAATC |
| Internal bar code: | TATTAGATGAGTTATGAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4125 |
| LEAP-Seq percent confirming: | 24.2424 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACACCATGAATGCCTGGG |
| Suggested primer 2: | GATGGTGACCTTCGAGCCAA |