Insertion junction: CLIP2.067976_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.067976
Chromosome:chromosome 16
Location:3594800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670973 GSTS3,GST3 (1 of 3) 2.5.1.18//5.3.99.2 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase // Prostaglandin-D synthase / Prostaglandin-H(2) Delta-isomerase; Glutathione S-transferase, similar to Prostaglandin-D Synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTAAGCACACGCCTCTATGTTGGGGGCCTTTGGCATCCCCGCAAATCCG
Internal bar code:CGCAGCCCCACTCTGGATTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4335
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTGCAACTGCCCCATA
Suggested primer 2:CTGCTGCCCTGTGAATCTGA