| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067986 |
| Chromosome: | chromosome 16 |
| Location: | 7911326 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690095 | (1 of 2) IPR000742//IPR017853 - EGF-like domain // Glycoside hydrolase superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTCGTGTCAAGTCAGCCACGTGGTTGTCGCGACCCTGCCATCTCCCC |
| Internal bar code: | TATCGTGTTCAAAATCCGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAGTAGACAGCAGGTGT |
| Suggested primer 2: | AAGACACCACCACTCCGTTC |