| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067993 |
| Chromosome: | chromosome 3 |
| Location: | 7564336 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205000 | IDA3 | Inner Arm Adaptor to IFT 3; (1 of 1) PF15669 - Coiled-coil domain-containing protein 24 family (CCDC24) | 3'UTR |
| Cre03.g205050 | CGLD24,DOT1,DGTT4 | Diacylglycerol acyltransferase, DGAT Type 2; (1 of 4) K14457 - 2-acylglycerol O-acyltransferase 2 (MOGAT2, MGAT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTAAAACTGTTAGTGCTGGTGTGGAAGGCTGGAGCCCGGAGGGCACGT |
| Internal bar code: | CGCTGTTCCAGTTAAACTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2179 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGGACGGGGCTGTACAT |
| Suggested primer 2: | TGTGGAGGTAGGAGATGGGG |