Insertion junction: CLIP2.067994_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067994
Chromosome:chromosome 17
Location:3465818
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g723700 (1 of 2) PTHR32246:SF18 - C2 DOMAIN-CONTAINING PROTEIN-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAATTATGCTTATCGGGGCTCAAGTCGCGAAATTGACCGAAGCCCAT
Internal bar code:GTGTGCCAGTGAGCTCCCTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3583
LEAP-Seq percent confirming:94.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAACCTGCGTGGCATATG
Suggested primer 2:CTGCCTTAAGGGTCTGGTGG