Insertion junction: CLIP2.067998_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.067998
Chromosome:chromosome 6
Location:5902881
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g289800 CAM12 Calmodulin-like protein; (1 of 1) 2.3.1.23//2.3.1.51//2.3.1.67 - 1-acylglycerophosphocholine O-acyltransferase / Lysolecithin acyltransferase // 1-acylglycerol-3-phosphate O-acyltransferase // 1-alkylglycerophosphocholine O-acetyltransferase / Platelet-activating factor-synthesizing enzyme 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTTGTATGGAATGGGCGCGCTGAGTGCACGGGGTAAAAGTCGCCGCA
Internal bar code:AGATTAACTAAGGTTGATAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2076
LEAP-Seq percent confirming:86.3636
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGAGTGGTGGAAAGTGTA
Suggested primer 2:TCAGACAGCAGCCTTTGAGG