| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068002 |
| Chromosome: | chromosome 10 |
| Location: | 6643486 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466200 | CYCAB1 | A/B-type cyclin; (1 of 3) K06627 - cyclin A (CCNA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTGGCGTCGATTATGGAGACGAGCCTTGCCTCTGGGAAGCGCCTGGC |
| Internal bar code: | TCCAATCGGAATTGTCAACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1723 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACCCCCTCTCAATGTC |
| Suggested primer 2: | TTTCTGTGACAGCGAGGTCC |