| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068014 |
| Chromosome: | chromosome 1 |
| Location: | 7018654 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050150 | NFO1 | NADH:flavin oxidoreductase/NADH oxidase; (1 of 1) 1.6.99.1 - NADPH dehydrogenase / NADPH diaphorase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGGACACAGCAAAAGACCAGAGTGGTACCCGCGTGCCTCCGCCGAC |
| Internal bar code: | ACGGCTGACAGGGGACTCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3904 |
| LEAP-Seq percent confirming: | 66.875 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 160 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTGTTGCCCGTATGT |
| Suggested primer 2: | GACGGCACTAGATACAGGCC |