| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.068023 |
| Chromosome: | chromosome 1 |
| Location: | 3355321 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021750 | (1 of 1) PF07818 - HCNGP-like protein (HCNGP) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCAAATGCGACAGTAGGTGGCACTCGCACCGCCGTGGTGAAGATCC |
| Internal bar code: | AGGCTAGGCCTACTTTAGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGCCCCGCAGATAACCC |
| Suggested primer 2: | CATGTTATGACTGCGCCGTG |