| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.068037 |
| Chromosome: | mitogenome |
| Location: | 2683 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreMt.g802338 | nad4,801494,ChrepMp02 | NADH dehydrogenase subunit 4; (1 of 1) K03881 - NADH-ubiquinone oxidoreductase chain 4 (ND4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGAAACCCAATAGATCCAAGACGCACAAGGCACTAAGTACTAGAACC |
| Internal bar code: | TCGGTTTTGCTGGTTGATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 19.2308 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGGCTATTGGTGACCG |
| Suggested primer 2: | CAACACGGTTGAAAGCCCAG |