| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068046 |
| Chromosome: | chromosome 1 |
| Location: | 7765374 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054750 | (1 of 37) 2.7.11.17 - Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGATGGATCCTGTCATGCACGCCCGTCACACTGCGCTTCGCAGCTAT |
| Internal bar code: | AGAAAGTTCTCTTTTAGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3720 |
| LEAP-Seq percent confirming: | 82.8125 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAACCTGGCAACCAAGC |
| Suggested primer 2: | GTGACAGGGGCTCACGTTAA |