| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068047 |
| Chromosome: | chromosome 6 |
| Location: | 3868625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278184 | (1 of 8) PTHR26379:SF144 - BTB/POZ DOMAIN-CONTAINING PROTEIN 19 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATGCTCCAAACTATGTCCATGCGAGTATGCTCACGTTACTTATTTAC |
| Internal bar code: | GCTTTCGAATGTAGTGCATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3464 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACCAGAGTCCGTCGTG |
| Suggested primer 2: | TGTGGTGCTTGCGTTTGATG |