| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068049 |
| Chromosome: | chromosome 16 |
| Location: | 6755512 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672602 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGCGCATGTTTCCGCCCGCATATTGGCATCCGTTAGTCGCTTTACA |
| Internal bar code: | GGAAGGCGCTCGGTGCCAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2362 |
| LEAP-Seq percent confirming: | 89.011 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGTCCCATCACACTTCC |
| Suggested primer 2: | TGTGGTGGGCCTAGATCGTA |