| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068059 |
| Chromosome: | chromosome 7 |
| Location: | 1274134 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322100 | UBQ5 | Ubiquitin fusion protein; (1 of 1) PF13019 - Telomere stability and silencing (Telomere_Sde2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGTGACTATTCGTACCGCCGCATTCGTTTGTGACATTGTCAAGATG |
| Internal bar code: | TTTCGCCTGTTGTAGTTCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2242 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGGTGACACAACAGACA |
| Suggested primer 2: | AGGACGGGAGGAGAATTCGA |