| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068061 |
| Chromosome: | chromosome 3 |
| Location: | 6646690 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGTGAGGTTGCAAAGGGGAGAACCACACCGGCGGGTGGGGGGACCAC |
| Internal bar code: | ACACGCGCTCGCCTTCTGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2346 |
| LEAP-Seq percent confirming: | 91.0112 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGCTGCCAGATCCTCC |
| Suggested primer 2: | TTCTCAAGCGTCACTGCCAT |