| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068067 |
| Chromosome: | chromosome 11 |
| Location: | 894076 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467652 | RAA9 | component psaA trans-splicing sub complex I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCAGGAGTTCGATGAAGAACTGTTTGGCGGCAGTAGCAGCAGAAGAC |
| Internal bar code: | CATGGGGTGCGTGATACGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGTCATCTCCGATGAGG |
| Suggested primer 2: | CTACCCTTTTCAGCCCCTGG |