Insertion junction: CLIP2.068072_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.068072
Chromosome:chromosome 2
Location:7668201
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147450 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGACTCGCGAGGTCGCTTCTCGCTCCAGCCGAGTTTTCCCTGCCGCC
Internal bar code:CGAAAGTCTAAGTTATTGCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2763
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGCTTACTTGGGAAGGT
Suggested primer 2:CTTGCAAGGGAAAAGTGGGC