Insertion junction: CLIP2.068091_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.068091
Chromosome:chromosome 9
Location:3648194
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g397068 Cytochrome P450, CYP3 superfamily 5'UTR
Cre09.g397105 (1 of 19) 1.14.14.1 - Unspecific monooxygenase / Xenobiotic monooxygenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGCTTAGGTTTAGCTAGGCGGGGAACTTCGGGCCTTGGATCCAAGT
Internal bar code:AAACAATTGCCCTATAAATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2084
LEAP-Seq percent confirming:96.5909
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:ACGATGTTCTCCGTGTGCTT
Suggested primer 2:AAAAATCCCAATGCCCGTGC