| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.068110 |
| Chromosome: | chromosome 5 |
| Location: | 718856 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245350 | (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGTGCCTGCACATATCACACACTCACACACTCACACATCAGCACACG |
| Internal bar code: | AGTCCAACTTTGCACCTGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGCATCTCCCCAATCG |
| Suggested primer 2: | CTACGGATGTAGGTTCCCGC |