Insertion junction: CLIP2.068119_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.068119
Chromosome:chromosome 8
Location:2018355
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g368700 RMT4 Putative protine N-methyltransferase; (1 of 1) PTHR13271//PTHR13271:SF40 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAACCCACTGGCCCGTAATAACACGTGCGCAAAGTGTGAGAGAAGATC
Internal bar code:GACCTCGAACACCAGCATCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3520
LEAP-Seq percent confirming:99.0196
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:102

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTTGCCGATGAGAACGT
Suggested primer 2:TAGGAGAGGCAATGGGACGA