| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068121 |
| Chromosome: | chromosome 6 |
| Location: | 2271171 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267100 | SRP68 | (1 of 1) K03107 - signal recognition particle subunit SRP68 (SRP68); Subunit of the Signal Recognition Particle | 5'UTR |
| Cre06.g267150 | (1 of 1) PTHR21231:SF8 - GPN-LOOP GTPASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGATGCTTTACCCAGACCGGCGTGGAACTCGTTCAGCGTCGACCGGGA |
| Internal bar code: | AGGATACGAACATCCGATACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAAGGCATGGCTGGTTT |
| Suggested primer 2: | CACCCCGTGCTTCTGTACTT |