Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.068123 |
Chromosome: | chromosome 8 |
Location: | 1393462 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g364200 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCCAGCGATGTCACCAACCACCAATTCCATGGGTTTCATTGCCATGC |
Internal bar code: | TCAGGGCTAGTATGACTCGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2074 |
LEAP-Seq percent confirming: | 12.0 |
LEAP-Seq n confirming: | 6 |
LEAP-Seq n nonconfirming: | 44 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACAGTTCCGCAGTGTTG |
Suggested primer 2: | CTCAAGGCGAATCCCCTGTT |