| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068132 |
| Chromosome: | chromosome 15 |
| Location: | 1562163 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750247 | (1 of 1) 5.2.1.12 - Zeta-carotene isomerase / 15-cis-zeta-carotene isomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCTTCATTGCCCCGGCCACATCACAGCCCTGAGAATCCCCACTCCCC |
| Internal bar code: | AGTACGACGCCAGAGTCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATTCCATTCACACCCA |
| Suggested primer 2: | AATCCAACCACGACCACCTC |