Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.068133 |
Chromosome: | chromosome 10 |
Location: | 3095175 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g441400 | NOP58 | (1 of 1) K14565 - nucleolar protein 58 (NOP58); Nucleolar protein, Component of C/D snoRNPs | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGCAGGGAGTGCGCCTTACTTAGGGACAACAATGAGATCGGCGTTGC |
Internal bar code: | TAGGACGGCACCGTCGAAAGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1975 |
LEAP-Seq percent confirming: | 97.8723 |
LEAP-Seq n confirming: | 46 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTCCCGATGTGTGTGGTTC |
Suggested primer 2: | ACGGCACTTACTAGGCCCTA |