| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068137 |
| Chromosome: | chromosome 2 |
| Location: | 2417626 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGGCCCAACTAAGCGAGGCCCAGATGCCCAACTAAGCGAGAGCTGCT |
| Internal bar code: | GTCCGTCAGGGACGCTAACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2910 |
| LEAP-Seq percent confirming: | 2.77778 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGCGAGCTTCAAAGACA |
| Suggested primer 2: | CTGCCAGTTCGGGTCAATCT |