| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068145 |
| Chromosome: | chromosome 13 |
| Location: | 2746756 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582150 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | 3'UTR | |
| Cre13.g582201 | (1 of 1) PTHR13622//PTHR13622:SF8 - THIAMIN PYROPHOSPHOKINASE // THIAMIN PYROPHOSPHOKINASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATACCGCAGCCGATGCTTAATGGCTAGTGTTGCCTCCCCAGTGCCCA |
| Internal bar code: | TGATTCATTGCGATGTGGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGTGAGGCGTAGTGA |
| Suggested primer 2: | GTCACGGATACACGCATTGC |