| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068151 |
| Chromosome: | chromosome 6 |
| Location: | 1233330 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258583 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCTGCCATAAACCATATTTCATCGCGTTCTTACTTTGCCGCGGGAT |
| Internal bar code: | GTACAGGGGAACTGAACCAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1471 |
| LEAP-Seq percent confirming: | 13.1148 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACTGAGAGCAAAGCCAC |
| Suggested primer 2: | TGCCGCATCTACCTTGTTGT |