| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068159 |
| Chromosome: | chromosome 7 |
| Location: | 3638878 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337250 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGGTCACTTGGCTGCCACACACTCGGATGTGACTGGTGAGTTGTTGC |
| Internal bar code: | CGCGACATGGTTATGTAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2986 |
| LEAP-Seq percent confirming: | 35.2941 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTGTGTGCATGTGTC |
| Suggested primer 2: | CGCATCAGATGGTGCATGTG |