Insertion junction: CLIP2.068165_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.068165
Chromosome:chromosome 12
Location:3875704
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515250 ROC7 Rythm Of Chloroplast bioluminescence 7; (1 of 1) PF09758 - Uncharacterised conserved protein (FPL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGGGCGGTGGCGCGTGCGGGGAGAGAGACAAGGCGCGTGGGCGTGT
Internal bar code:GTAATTGAATCCGATAATGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:966
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GAACAACGCACCTGACAACC
Suggested primer 2:CCACACCGGAACTGTGATCA