| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068168 |
| Chromosome: | chromosome 3 |
| Location: | 6539274 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194150 | (1 of 1) PF08698 - Fcf2 pre-rRNA processing (Fcf2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCAGTGAGCAGTTCTTCAGCGCAGCGCGGTTAGCTGTGAGGGGTAGGC |
| Internal bar code: | GTTCGTCGGAACGGCGAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1783 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGAAGCGAGCATTCTTG |
| Suggested primer 2: | TGTGTCCACAGAGCTTCGAC |