| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068175 |
| Chromosome: | chromosome 4 |
| Location: | 3085427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAGCCGTTACCCGCAGCCCGTCAGTGAGAGCCAAGGCCCCTGCCCAC |
| Internal bar code: | GCCTACAATGTTTGATCTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3342 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 140 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 160 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCTAGGCGGGTTGGATA |
| Suggested primer 2: | CCGCCTCTCTAAGCCTAAGC |