| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068178 |
| Chromosome: | chromosome 1 |
| Location: | 4960266 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034400 | PGL1 | (1 of 2) 3.1.1.31 - 6-phosphogluconolactonase; 6-phosphogluconolactonase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCACAGCGACAGCTGCCTACTATGCTGATCCCCAATGTCGGGCCAGC |
| Internal bar code: | GAGTCATGTAGCGTTTGCAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2576 |
| LEAP-Seq percent confirming: | 85.1852 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGCTCAAGTGGATCCT |
| Suggested primer 2: | CCGCTGTCTCACCACTTGAT |