| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068184 |
| Chromosome: | chromosome 3 |
| Location: | 1660381 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152900 | MPA5 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCGCTGCGAATGCGTCCGAGTACTTGAATGGCTAAGAGCTGGGTCAC |
| Internal bar code: | CGACCTGGAGGACGGCTATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3302 |
| LEAP-Seq percent confirming: | 68.1818 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGGCATGTGTGTGGTG |
| Suggested primer 2: | ACCACGGAACATCGAAGCTT |