| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068186 |
| Chromosome: | chromosome 3 |
| Location: | 8539263 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g211297 | 5'UTR | ||
| Cre03.g211409 | PPR15,mS106,MRPS106 | Mitochondrial ribosomal protein S106, Chlamydomonas specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGAGAGCTGTGGCCATTCCCATCGCCGCACCCGTCTAGGGGTTCTGC |
| Internal bar code: | CTACCAAAGAGAGTAAGGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3811 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTGTACTTATCGGCCCG |
| Suggested primer 2: | TTGACGCCCTTCCTGGTTAC |