| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068190 |
| Chromosome: | chromosome 9 |
| Location: | 2004514 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396800 | CSE19 | Predicted protein of CSE family | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGAAAGCATCAATGGATTTCTGTTGGAGTCCATCATCCCTGGAGGCT |
| Internal bar code: | GTGCCGGTCGTAATAGCAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 81.4815 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCTGGCAGTTGGAGTGG |
| Suggested primer 2: | GCCACCGACGTGTTCTTAGA |