| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068209 |
| Chromosome: | chromosome 16 |
| Location: | 7897826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689871 | TSI1 | (1 of 1) PTHR11946:SF82 - ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; Putative organellar isoleucyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGCCCAGTGCTGACCTACCTGAGCCACATCCACCCAGCACCCCAGC |
| Internal bar code: | TGGAGGGCGATCATTCGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1350 |
| LEAP-Seq percent confirming: | 85.2941 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGAGGGAATCGCACTGG |
| Suggested primer 2: | GGGTGCCTCCTCTACAGAGA |