| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068219 |
| Chromosome: | chromosome 17 |
| Location: | 2015819 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAATGGACCCCGAGAAACACGGACCGCCAAGCAACACAATGCAGCCGC |
| Internal bar code: | CACAGATGAGCTGGTAGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4464 |
| LEAP-Seq percent confirming: | 68.6274 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAAAGGTGTTGGCTTGG |
| Suggested primer 2: | CGTCACTACCGTCACACACA |