Insertion junction: CLIP2.068227_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.068227
Chromosome:chromosome 3
Location:585429
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145367 (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) 5'UTR
Cre03.g145387 FAP239 Coiled-Coil Flagellar Associated Protein 239 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCACGCACGCATGGACTGCTGGACAGTCACACAGCCCAGGCCTTCTG
Internal bar code:CGGGCTGCGCCGAGTCGCAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4822
LEAP-Seq percent confirming:85.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTAGCACTAGTCGATGT
Suggested primer 2:GGCAATTCTAGGGCAGTGGT