| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068236 |
| Chromosome: | chromosome 2 |
| Location: | 1279497 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082250 | HSK2 | (1 of 1) K00872 - homoserine kinase (thrB1); Homoserine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTACGATGCCACGCACGCAACGCGGTGACGTGGTACTCTTCATTTTGC |
| Internal bar code: | CTTATGTGGAACCCGCGGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2813 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACGTCAAGAAGCCTCT |
| Suggested primer 2: | CGGTGATTTTGGTGCTTGCA |