| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068240 |
| Chromosome: | chromosome 12 |
| Location: | 5215962 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527200 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGATATGTGCACCGCTTGTTGGAGGCTGTTCGGCCCGAAAGCTTGAA |
| Internal bar code: | TGAATTCTCATCTCGGCCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2680 |
| LEAP-Seq percent confirming: | 55.2083 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAATGTGGTCGTGGCTGG |
| Suggested primer 2: | GCAACCTAGTCACCGGGTAC |