| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068252 |
| Chromosome: | chromosome 16 |
| Location: | 1419544 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652300 | CMA2,TTL13 | Chlamydomonas-specific membrane protein family A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGAAATGGGATCCAAGGGACGCCAAATCACTCGTTCTCGCCCGCGCA |
| Internal bar code: | GTCCGGCTGCAAGTGGCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3092 |
| LEAP-Seq percent confirming: | 31.6832 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGACGACCTTTCCCTGTG |
| Suggested primer 2: | TCCTGCGATGACGTTTCCAA |