| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068255 |
| Chromosome: | chromosome 6 |
| Location: | 3047749 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274700 | OTU4 | (1 of 2) K13719 - ubiquitin thioesterase OTU1 (OTU1, YOD1); OTU-like cysteine protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCGGGAGGTGCATGGGAATGGTGTAGTAGTACGCAATTGGGGGCCCG |
| Internal bar code: | GAAATACTTTCCCCCGTACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4219 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTCACCTCGGGCTAAGC |
| Suggested primer 2: | ATAATGGTTCAGGAGCCGCC |