Insertion junction: CLIP2.068272_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.068272
Chromosome:chromosome 10
Location:6584183
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465700 (1 of 1) PF01082//PF03712 - Copper type II ascorbate-dependent monooxygenase, N-terminal domain (Cu2_monooxygen) // Copper type II ascorbate-dependent monooxygenase, C-terminal domain (Cu2_monoox_C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTTGCCCTCAGACCGCGCTGTCCGGCGCCCTGTCCCGCATCTCCGGCC
Internal bar code:CGGTGGTTCATATAAACATACG

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTGTCTCCGGACCTGAG
Suggested primer 2:CGTACATGCTGCTCCATTGC