| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068285 |
| Chromosome: | chromosome 16 |
| Location: | 3644941 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675602 | (1 of 1) K12669 - oligosaccharyltransferase complex subunit gamma (OST3, OST6) | 3'UTR/3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCATCAGTAAACCATCTGCACCCAGTTCAGTGACCCCGGGTCACTTG |
| Internal bar code: | CCTTCTTGTTCATTAGACTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1555 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGAAGATAGACGCGTCC |
| Suggested primer 2: | GACTGGCCGCTCATACTACC |