| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068286 |
| Chromosome: | chromosome 12 |
| Location: | 2720898 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504850 | HFO16 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTTTGGTGTTGCGGGTCACAGGGACCGCAACAGTGTGCGCAGCGTGC |
| Internal bar code: | ATACCAGGCCACACGGCTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3073 |
| LEAP-Seq percent confirming: | 79.7872 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTGAGAACGATCGGAG |
| Suggested primer 2: | GCCTCTGCTCAAGTCTGTGT |