| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.068288 |
| Chromosome: | chromosome 12 |
| Location: | 4431786 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520100 | CYG66 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGAAGTCAGCAACACTTGTCGGAGCTGCGAGCAAACGACAGGAAGTA |
| Internal bar code: | TAGTTGGAAATAAATATGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3163 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACCGAATGAAAGGACGA |
| Suggested primer 2: | CTGTGCAATAACACCTCGCG |