| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068292 |
| Chromosome: | chromosome 10 |
| Location: | 6177814 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462700 | (1 of 1) K14397 - cleavage and polyadenylation specificity factor subunit 5 (NUDT21, CPSF5, CFIM25) | 3'UTR | |
| Cre10.g462750 | (1 of 1) K11374 - elongator complex protein 2 (ELP2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGGCGGCGGCCTGGGAGCTGGGCCAGGGTCGCCGCGTTGTGGAGCG |
| Internal bar code: | CGGGGTAAATCGTCCTTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1735 |
| LEAP-Seq percent confirming: | 1.1236 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGGCAAGAGGAGAAAGG |
| Suggested primer 2: | GTTTGACCTGCACGACAACC |