Insertion junction: CLIP2.068312_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.068312
Chromosome:chromosome 7
Location:2704960
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g331050 (1 of 1) IPR000104//IPR001487 - Antifreeze protein, type I // Bromodomain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTAGCGTGTTGAATAGGCTGGCGTAAGTTGTGAACGTGGGGAGACGG
Internal bar code:GTTAAACGGTGCGGGATAGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:302
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGACGACTACTACCGC
Suggested primer 2:GCGTGTGTCATGTTGCTTGT